Ordering information
Name	Cat. No.	Vol.	Scheme
G-Alkyne	PMG034-2	2 ml

1. Overview

Click Chemistry describes rapid, selective "click" reactions between paired functional groups in mild aqueous solutions. This concept has evolved into a convenient, versatile, and reliable two-step coupling procedure for molecules A and B, widely adopted in biosciences, drug discovery, and materials science.

PuriMag™ G-Alkyne Alkyne-Functionalized Magnetic Nanoparticles are uniform, polymer-coated superparamagnetic nanoparticles. Their hydrophilic surface ensures excellent dispersibility, low non-specific adsorption, and easy handling in diverse buffers. The beads feature a high-density surface coating of alkyne functional groups, enabling covalent capture of functionalized proteins via Cu(I)-catalyzed Azide-Alkyne Cycloaddition (CuAAC).

Workflow Requirements:

    Target proteins must be metabolically, enzymatically, or chemically tagged with azide groups.

    Post-coupling rigorous washing eliminates non-specifically bound proteins.

    Unique surface chemistry ensures ultra-low background binding without surfactants.

Features & Advantages:
    High binding capacity

    Rapid, efficient coupling
    Charge-neutral surface post-coupling
    Stable covalent bonds with minimal ligand leakage
    Low non-specific binding
    Capacity: 1–20 mg protein or 0.1–2 mg peptide per gram beads

Caution: Alkyne beads are unstable in organic solvents and high urea concentrations.

Schematic Diagram of Coupling Mechanism:

2. product description

Product Specifications
Description	Polymer coated Fe3O4 nanoparticles
Particle Size	200 nm
Number of Beads	~1.7×1010 beads/mg
Matrix	Proprietary polymer
Functional group	Alkyne group
Group density	~100 µmole / g of Beads
Magnetization	60~70 EMU/g
Formulation	10mg/mL in DI water
Storage	1 year at 2~8 ℃. Do not freeze.

3. Instructions for Use

    1.Add ~10 mg PuriMag™ Alkyne Magnetic Nanoparticles to 25 mL deionized water.

    2.After brief ultrasonication, add:

            5 mg copper sulfate solution (5 mg in 0.5 mL H₂O)

            22 mg sodium ascorbate solution (22 mg in 1 mL H₂O)

            Stir mixture for 30 min.

    3.Add azide-functionalized compound, stir overnight at RT.

    4.Recover product via magnetic separation. Wash with:

            Distilled water

            PBS (pH 7.0)

    5.Treat final product with EDTA to remove copper ions.

            (For research use only!)