Ordering information
Name	Cat. No.	Vol.	Scheme
G-Botin	PMG016-2	2 ml

1. Overview

PuriMag™ G-Biotin are biotin-conjugated magnetic nanoparticles designed for the magnetic isolation of avidin-, neutravidin-, or streptavidin-labeled components. These beads feature a large surface area and deliver high capture efficiency in binding assays.

(Note: Ideal for pull-down assays, diagnostic applications, and target purification workflows requiring biotin-streptavidin affinity systems.)

2. product description

Product Specifications
Description	Polymer coated Fe3O4 nanoparticles
Particle Size	200 nm
Number of Beads	~1.7×1010 beads/mg
Matrix	Proprietary polymer
Functional group	Streptavidin group
Group density	~150 μmol biotin / g Beads
Binding capacity	≥30 μg streptavidin / mg Beads
Magnetization	60~70 EMU/g
Formulation	10mg/mL in 25 mM tris-HCl,  pH 7.4
Storage	1 year at 2~8 ℃. Do not freeze.

3. Instructions for Use

A. Materials Provided
Biotin Magnetic Beads, 10 mg/mL

B. Additional Materials (Not Provided)

1. Binding/Wash Buffer: TBS with 0.05% Tween-20

2. Elution Buffer: 8M Guanidine HCl, pH 1.5

C. Isolation Procedure

1. Pipette 50 μL (0.5 mg) beads per tube. Add 1 mL Binding Buffer to wash beads.

2. Perform magnetic separation for 2 min or until supernatant clears.

3. Aspirate and discard supernatant. Add 1 mL Binding Buffer for a second wash.

4. Repeat Step 2. Discard supernatant.

5. Resuspend beads in 450 μL Binding Buffer.

6. Add 50 μL serum or cell culture supernatant to beads.
   Note: Sample volume may be modified. If <500 μL, dilute to 500 μL final volume with Binding Buffer.

7. Mix gently via vortex or rotator for 30 min.

8. Separate magnetically for 2 min or until supernatant clears.

9. Remove and discard supernatant.

10. Add 500 μL Binding Buffer to wash away unbound proteins.

11. Repeat Steps 8-9. Discard supernatant.

12. Add 100 μL Elution Buffer to beads and mix thoroughly.

13. Incubate at RT for 10 min with occasional gentle agitation/vortexing.

14. Desalt or dialyze eluted samples into an appropriate buffer.

(For research use only!)