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客户采用G-SA链霉亲和素磁珠偶联核酸适配体发表SCI论文2篇

2023-9-24 23:03:13点击:

He, Y., et al. Selected ssDNA aptamers–graphene oxide‐based fluorescent biosensor to detect sulfameter in milk, Luminescence 2023, 38(5), 518. https://doi.org/10.1002/bio.4473 SA磁珠偶联适配体)

 

Selected ssDNA aptamers–graphene oxide-based fluorescent biosensor to detect sulfameter in milk

选定基于ssDNA适配体-氧化石墨烯的荧光生物传感器用于检测牛奶中的磺胺对甲氧嘧啶

The abuse of sulfameter (SME) in animal husbandry can cause drug resistance and toxic or allergic reactions in humans. Therefore, it is very important to establish a simple, inexpensive, and efficient method for detecting SME in food. In this work, we propose a single fluorescent aptamer/graphene oxide (GO)-based biosensor to detect SME residues in milk. Aptamers that specifically bind to SME were screened using capture-SELEX and a ssDNA library immobilized on magnetic beads. The 68 active candidate aptamers were chemically synthesized for specificity and affinity characterization. Among the aptamers, the aptamer sulf-1 revealed the highest affinity (Kd = 77 ± 15 nM) to SME and was selected to construct a GO-based fluorescent biosensor for real milk sample detection. Under optimal conditions, the single fluorescent aptasensor had a wide linear range (R2 was 0.997) from 7 to 336 ng/ml and a low detection limit of 3.35 ng/ml that was calculated with a 3SD/slope. The single fluorescent method was also validated using SME-fortified milk samples, showing average recoveries ranging from 99.01% to 104.60% with a relative standard deviation of less than 3.88%. These results demonstrate that this novel aptamer sensor provides an opportunity for sensitive, convenient, and accurate detection of SME residues in milk.

在畜牧业中滥用磺胺对甲氧嘧啶(SME)会导致人类产生耐药性和毒性或过敏反应。因此,建立一种简单、廉价、高效的食品中SME检测方法非常重要。在这项工作中,我们提出了一种基于荧光适体/氧化石墨烯(GO)的生物传感器来检测牛奶中的SME残留。使用捕获SELEX和固定在磁珠上的ssDNA文库筛选与SME特异性结合的适配体。对68种活性候选适配体进行化学合成,用于特异性和亲和力表征。在核酸适配体中,适配体硫-1对SME的亲和力最高(Kd = 77 ± 15 nM),被选中构建用于真实牛奶样品检测的GO荧光生物传感器。在最佳条件下,单个荧光适体传感器具有7至336 ng/ml的宽线性范围(R2为0.997),使用3SD/斜率计算的检测限低至3.35 ng/ml。使用SME强化牛奶样品也验证了单一荧光方法,平均回收率范围为99.01%至104.60%,相对标准偏差小于3.88%。这些结果表明,这种新型适配体传感器为灵敏、方便和准确地检测牛奶中的SME残留提供了机会。



Jinru Zhou, et al. A ratiometric fluorescent aptasensor based on EXPAR to detect shellfish tropomyosin in food system, Food Control, Volume 144, 2023, 109380,

https://doi.org/10.1016/j.foodcont.2022.109380.  SA磁珠偶联适配体)



A ratiometric fluorescent aptasensor based on EXPAR to detect shellfish tropomyosin in food system


摘要
贝类过敏是一个关键的公共卫生问题,因此迫切需要一种准确、简单和可靠的方法来量化复杂食物系统中的贝类过敏原。原肌球蛋白(TM)被称为贝类的主要过敏原。本文开发了一种以TM特异性适配体为识别探针的新型开关转换比例荧光生物传感器,用于检测复杂食品系统中的TM。所提出的适体传感器对食品中TM的定量表现出良好的准确性、稳定性和选择性。此外,使用七种不同的贝类样品验证了该适体传感器在复杂食品样品中的适用性。因此,这种比例式荧光适体传感器能够准确检测食品中TM痕量的存在,为过敏原标签监督提供技术支持。


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