客户采用我司G-ProA蛋白A磁珠偶联抗体在Current Microbiology发表论文-生物磁珠专家

客户采用我司G-ProA蛋白A磁珠偶联抗体在Current Microbiology发表论文

2023-9-24 22:51:57点击：

Zheng, H., et al. Establishment of a Fast Diagnostic Method for Sepsis Pathogens Based on M1 Bead Enrichment. Curr Microbiol 80, 166 (2023). https://doi.org/10.1007/s00284-023-03280-6 (G-ProA蛋白A磁珠偶联抗体)

Establishment of a Fast Diagnostic Method for Sepsis Pathogens Based on M1 Bead Enrichment

基于M1磁珠富集的脓毒症病原菌快速诊断方法的建立

Hao Zheng1 · Xiaoli Chen1 · Wenge Li 1 · Jinxing Lu1 · Xiaoping Chen1

Received: 22 May 2022 / Accepted: 21 March 2023 / Published online: 6 April 2023 © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2023

Abstract

Blood culture-based sepsis diagnostic methods usually cannot obtain positive results in a timely manner. Molecular diagnostic methods, such as real-time PCR without blood culture, would be more time-saving and suitable for pathogenic diagnosis of sepsis, while their sensitivities have always been unsatisfactory for the usually low concentration of pathogens in the blood of sepsis patients. In this study, we established a fast diagnostic method using magnetic beads coated with human recombined mannose-binding lectin that makes it possible to concentrate pathogens from human plasma that have low concentrations of pathogens. With subsequent microculture (MC) and real-time PCR, this method allowed the detection of 1–10 CFUs/ ml of Staphylococcus aureus, Group A Streptococcus, Escherichia coli, Pseudomonas aeruginosa, Candida tropicalis, or

C. albicans from human plasma within 9.5 h, which was 21–80 h earlier than blood culture. The combination of pathogen enrichment and MC made the detection of sepsis-causing pathogens more time-saving and more sensitive than blood culture or real-time PCR alone.

摘要：
基于血培养的脓毒症诊断方法通常不能及时获得阳性结果。分子诊断方法，如无血培养的实时荧光定量PCR，更省时，适合于脓毒症的病原诊断，而对于脓毒症患者血液中通常低浓度的病原体，其敏感性一直不尽如人意。在这项研究中，我们建立了一种快速诊断方法，使用涂有人重组甘露糖结合凝集素的磁珠，该方法可以从具有低浓度病原体的人血浆中浓缩病原体。通过随后的显微培养（MC）和实时荧光定量 PCR，该方法可检测 1-10 CFU/ml 金黄色葡萄球菌、A 组链球菌、大肠杆菌、铜绿假单胞菌、热带念珠菌或
9.5小时内从人血浆中取出白色念珠菌，比血培养早21-80小时。病原体富集和MC的结合使得检测引起脓毒症的病原体比单独的血培养或实时荧光定量PCR更省时，更灵敏。

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