联系方式 Contact

地址:厦门市集美大道1300号

电话:15805933710

联系人:许先生

QQ:1974707632

微信:15805933710

邮件:purimag@139.com

在线QQ交谈 在线QQ交谈

搜索 Search

客户采用我司链霉亲和素磁珠PuriMag G-Strep在ACS Applied Materials & Interfaces ( IF 8.758 ) 发表文章

2021-5-17 21:16:52点击:

客户采用我司链霉亲和素磁珠PuriMag G-Strep偶联适配体,开发了一种低成本的检测方法。

Facile, Rapid, and Low-Cost Electrophoresis Titration of Thrombin by Aptamer-Linked Magnetic Nanoparticles and a Redox Boundary Chip.


ACS Applied Materials & Interfaces ( IF 8.758 ) Pub Date : 2019-08-08 , DOI: 10.1021/acsami.9b09598
Hao Kong,Wei-Wen Liu,Wei Zhang,Qiang Zhang,Cun-Huai Wang,Muhammad Idrees Khan,Yu-Xing Wang,Liu-Yin Fan,Cheng-Xi Cao


An aptamer-linked assay of a target biomarker (e.g., thrombin) is facing the challenges of long-term run, complex performance, and expensive instrument, unfitting clinical diagnosis in resource-limited areas. Herein, a facile chip electrophoresis titration (ET) model was proposed for rapid, portable, and low-cost assay of thrombin via aptamer-linked magnetic nanoparticles (MNPs), redox boundary (RB), and horseradish peroxidase (HRP). In the electrophoresis titration-redox boundary (ET-RB) model, thrombin was chosen as a model biomarker, which could be captured within 15 min by MNP-aptamer 1 and HRP-aptamer 2, forming a sandwich complex of (MNP-aptamer 1)-thrombin-(HRP-aptamer 2). After MNP separation and chromogenic reaction of 3,3',5,5'-tetramethylbenzidine (TMB) within 10 min, an ET-RB run could be completed within 5 min based on the reaction between a 3,3',5,5'-tetramethylbenzidine radical cation (TMB•+) and l-ascorbic acid in the ET channel. The systemic experiments based on the ET-RB method revealed that the sandwich complex could be formed and the thrombin content could be assayed via an ET-RB chip, demonstrating the developed model and method. In particular, the ET-RB method had the evident merits of simplicity, rapidity (less than 30 min), and low cost as well as portability and visuality, in contrast to the currently used thrombin assay. In addition, the developed method had high selectivity, sensitivity (limit of detection of 0.04 nM), and stability (intraday: 3.26%, interday: 6.07%) as well as good recovery (urine: 97-102%, serum: 94-103%). The developed model and method have potential to the development of a point-of-care testing assay in resource-constrained conditions.


中文翻译: