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客户采用我司不同修饰硅磁珠研究核酸提取机理在《Separation and Purification Technology》发表SCI论文

2021-6-2 21:01:14点击:

客户采用我司不同修饰硅磁珠,研究了磁珠大小、磁珠表面基团在动物组织样本基因组提取过程中的影响,为核酸提取磁珠的选择使用提供理论支撑。

Peipei Li, Menghang Li, Fan Zhang, Mengyuan Wu, Xiaobin Jiang, Bo Ye, Zhenjun Zhao, Dongmei Yue, Qi Fan, Haixia Chen, High-efficient nucleic acid separation from animal tissue samples via surface modified magnetic nanoparticles,Separation and Purification Technology,Volume 262,2021,118348, 

Abstract
High efficiency nucleic acid (NA) separation via magnetic nanoparticles (MNPs) is a promising technology for bioengineering, healthcare, etc. Aimed to enhance the selection of MNPs and illustrate the separation mechanism for effective isolation of NAs from complex animal samples, this study fabricated hydroxy-, amino- and carboxyl-functionalized MNPs (MNPs-OH, MNPs-NH2 and MNPs-COOH) with different particle sizes to investigate the hybrid NA separation performance from animal tissue samples. Molecular dynamics (MD) simulation was introduced to predict the affinity action of MNPs with NA and typical impurities. The details of the kinetic separation behavior and the NA separation performance were then illustrated. MD simulation results showed MNPs-NH2 had a stronger affinity with NA, and in most experimental separation systems, NA separation yield via three kinds of MNPs showed a following order of MNPs-NH2 > MNPs-OH > MNPs-COOH. MNPs of different sizes had significantly different sedimentation and dispersion features, leading to diverse separation results. Combining with the proper lysis system, an optimized NA separation method at room temperature with high-throughput and low contamination risk was developed as a promising approach for large-scale samples extracted NA simultaneously. This work could provide a theoretical guidance for the fabrication strategy of advanced MNPs and the enhancement of NA separation from complex samples.


摘要
利用磁性纳米粒子(MNPs)高效分离核酸是生物工程、医疗保健等领域的一项很有前途的技术。为了提高MNPs的选择性,阐明从复杂动物样品中有效分离核酸的分离机理,本研究制备了羟基-,研究了不同粒径的氨基和羧基功能化MNPs(MNPs-OH、MNPs-NH2和MNPs-COOH)从动物组织样品中分离杂交NA的性能。采用分子动力学模拟方法预测了MNPs与NA及典型杂质的亲和作用。详细说明了动力学分离行为和钠分离性能。MD模拟结果表明,MNPs-NH2对NA有较强的亲和性,在大多数实验分离体系中,三种MNPs对NA的分离率依次为MNPs-NH2>MNPs-OH>MNPs-COOH。不同粒径的MNPs具有明显不同的沉降和分散特性,导致分离效果不同。结合合适的裂解体系,建立了一种高通量、低污染风险的室温钠分离方法,为大规模样品同时提取钠提供了一种新的途径。这项工作为先进MNPs的制备策略和提高复杂样品中NA的分离提供了理论指导。

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