常用缓冲液配制计算器

来源:生物磁珠专家 2020-8-20 16:47:47      点击:

实验室常用缓冲液配制,请点击下述链接,输入对应pH和盐浓度,即可得到详细配制方法。

Buffer

Min pH

Max pH

ACES Buffer (0.1 M, 6.7 pH)

6.1

7.2

Acetate Buffer (0.1 M, pH 5.0)

3.7

5.6

ADA Buffer (0.5 M, 6.6 pH)

6.0

7.2

BES-Buffered Saline (2X) (0.05 M, pH 6.95)

6.4

7.8

Bicine (1 M, pH 8.26)

7.6

9.0

Bis-Tris Buffer (1 M, 6.5 pH)

5.8

7.2

CAPS (0.5 M, pH 10.4)

9.7

11.1

CHES (0.5 M, pH 9.5)

8.6

10.0

Citrate Buffer (0.1 M, pH 6.0)

3.0

6.2

Citrate-Phosphate Buffer (0.15 M, pH 5.0)

2.6

7.6

Diethanolamine (1 M, pH 9.8)

7.8

9.9

EBSS (magnesium, calcium, phenol red) (pH 7.0)

7.0

7.4

Glycine-HCl Buffer (0.1 M, pH 3.0)

2.2

3.6

Glycine-Sodium Hydroxide Buffer (0.08 M, pH 10)

8.6

10.6

HBSS (Hank's Balanced Salt Solution)

7.2

7.6

HEPES Buffer (1 M, 7.5 pH)

6.8

8.2

HEPPSO (1 M, pH 7.85)

7.1

8.5

HHBS (Hank's Buffer with Hepes)

7.2

7.4

Hydrochloric Acid-Potassium Chloride Buffer (0.1 M, pH 2.0)

1.0

2.2

Imidazole-HCl Buffer (0.05 M, pH 7.0)

6.2

7.8

Maleic Acid (1 M, pH 7.5)

7.0

8.0

MES (0.5 M, pH 6)

5.5

6.7

MOPS Buffer (10X) (0.2 M, pH 7)

6.5

7.9

MOPSO Buffer (0.5 M, 6.9 pH)

6.1

7.6

PBS (Phosphate Buffered Saline) (1X, pH 7.4)

7.3

7.5

PIPES Buffer (1 M, 6.8 pH)

6.1

7.5

Sodium Borate Buffer (1 M, pH 8.5)

8.0

10.0

TAE (1 M, pH 8.6)

8.0

8.5

TBS (1 M, pH 7.4)

7.2

7.5

TE Buffer 10X

7.5

8.5

TES Buffer (1 M, 7.5 pH)

6.8

8.2

Tricine (1 M, pH 8.05)

7.4

8.8

Tris Buffer (1 M, pH 7.2)

7.0

7.5

Acetate Buffer (pH 3.6 to 5.6)

3.7

5.6

Carbonate-Bicarbonate Buffer (pH 9.2 to 10.6)

9.2

10.8

Citrate Buffer (pH 3.0 to 6.2)

3.0

6.2

Phosphate Buffer (pH 5.8 to 7.4)

5.8

8.0

Potassium Phosphate (pH 5.8 to 8.0)

5.8

8.0

Trizma® Buffer (pH 7.0 to 9.2)

7.2

9.0