文章快递：客户采用我司羧基磁珠偶联噬菌体蛋白实现细菌捕获和检测
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Ding Y, Yang Q, Liu X, et al. An ultrasensitive fluorescence nano-biosensor based on RBP 41-quantum dot microspheres for rapid detection of Salmonella in the food matrices[J]. Food Chemistry, 2025, 468: 142504.
An ultrasensitive fluorescence nano-biosensor based on RBP 41-quantum dot microspheres for rapid detection of Salmonella in the food matrices
一种基于 RBP 41 量子点微球的超灵敏荧光纳米生物传感器，用于快速检测食品基质中的沙门氏菌
AbstractSwift screening of Salmonella-contaminated food is crucial for timely prevention and control of foodborne illness outbreaks. A novel phage receptor binding protein (RBP 41) was previously identified and characterized from phage T102. This study functionalized RBP 41 onto magnetic beads (MBs) and quantum dot microspheres (QDMs) to form magnetic separation and fluorescent probes, respectively. The bacteria were captured by RBP 41-MBs and labelled with RBP 41-QDMs to form MBs-RBP 41-bacteria-RBP 41-QDMs complexes, then the fluorescence intensity of complexes was detected for determination of Salmonella. This proposed biosensor was demonstrated to detect Salmonella as low as 0.1245 Log10 CFU/mL (∼2 CFU/mL) within ∼1.5 h. The recovery yield of Salmonella in the spiked food samples ranged from 87 % to 119 %, indicating that it could detect Salmonella in real samples. This novel magnetic fluorescence nano-biosensor has potential to detect the bacteria in the different sample to reduce the detection time and increase sensitivity.
迅速筛查受沙门氏菌污染的食物对于及时预防和控制食源性疾病暴发至关重要。先前从噬菌体 T102 中鉴定并表征了一种新的噬菌体受体结合蛋白 （RBP 41）。本研究将 RBP 41 功能化到磁珠 （MB） 和量子点微球 （QDM） 上，分别形成磁性分离和荧光探针。用 RBP 41-MBs 捕获细菌，并用 RBP 41-QDMs 标记，形成 MBs-RBP 41-bacteria-RBP 41-QDMs 复合物，然后检测复合物的荧光强度，用于测定沙门氏菌。这种提出的生物传感器被证明可在 ∼1.5 小时内检测到低至 0.1245 Log10 CFU/mL （∼2 CFU/mL） 的沙门氏菌。加标食品样品中沙门氏菌的回收率为 87% 至 119%，表明它可以在真实样品中检测到沙门氏菌。这种新型磁性荧光纳米生物传感器有可能检测不同样品中的细菌，以减少检测时间并提高灵敏度。
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