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使用MagStart-Amine、MagStart-HILIC和MagStart-SAX三种磁珠对蛋白的酶解液除杂的简易流程

2024-5-15 23:38:12点击:

Conditions

MagStart-Amine (PAC)

Bind

50%~80% ACN or EtOHpH=8

Wash

80% EtOH and 95% ACN

Digest

LysC / Trp 1-4 hour

Elute

H2O or 0.5% TFA or DMSO

Range

1 μg~200 μg

Extration

SDSNP40Tween SDC

Clean-up of

Proteins

Fractionation

NA

Top-down

NA

MagStart-Amine磁珠购买请参考:

 胺基磁珠 MagStart-Amine——高结合量蛋白质组学前处理-生物磁珠专家 (purimagbead.com)


Conditions

MagStart-HILIC

Bind

15 CAN100 mM NH4AcpH=4.5

Wash

95% ACN

Digest

LysC / Trp 1-4 hour

Elute

1% TFA

Range

5 μg~0.5 mg

Extration

SDSNP40Tween SDC

Clean-up of

ProteinsPeptides & Glycans

Fractionation

NA

Top-down

NA

MagStart-HILIC磁珠购买请参考:

HILIC磁珠 MagStart-HILIC——高结合量蛋白质组学前处理-生物磁珠专家 (purimagbead.com)


Conditions

MagStart-SAX

Bind

100 mM Borate pH=10

Wash

10 mM Borate pH=10

Digest

LysC / Trp 1-4 hour

Elute

1% TFA frac pH102

Range

20 μg~5 mg

Extration

Urea

Clean-up of

Proteins & Peptides

Fractionation

Salt or pH Gradient

Top-down

Salt or pH Gradient

MagStart-SAX磁珠购买请参考:

SAX磁珠 MagStart-SAX——高结合量蛋白质组学前处理-生物磁珠专家 (purimagbead.com)