Name: Streptavidin（链霉亲和素） Description: Streptavidin Recombinant （重组链霉亲和素） Pricings: 5mg (RMB 500.00), 20mg (RMB 1080.00), 100mg (RMB 3280.00), 1g (RMB 24000.00) Shipped: At Room Temperature Catalogue Number: PRO-rSA001 INTRODUCTION Streptavidin is a tetrameric protein secreted by Streptomyces avidinii which binds firmly to biotin. Streptavidin is widely used in molecular biology through its unique high affinity for the vitamin biotin. The dissociation constant (Kd) of the biotinstreptavidin complex is about ~1015 mol/L. The strong affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. The streptavidin/biotin system has one of the biggest free energies of association of yet observed for noncovalent binding of a protein and small ligand in aqueous solution (K_assoc = 1014). The complexes are also extremely stable over a wide range of temperature and pH. DESCRIPTION Streptavidin Streptomyces Avidinii Recombinant produced in E.Coli. The molecular weight per tetramer is approximately 52kDa. SOURCE Escherichia Coli. PHYSICAL APPEARANCE Sterile Filtered White lyophilized (freezedried) powder. FORMULATION Lyophilized in 10mM potassium phosphate buffer pH 6.5. SOLUBILITY It is recommended to reconstitute the lyophilized Streptavidin in sterile 18MΩ-cm H2O not less than 0.5 mg/ml, which can then be further diluted to other aqueous solutions. STABILITY Streptavidin is shipped at ambient temperature, upon arrival store at 20 ℃. PURITY Greater than 98.0% as determined by SDS-PAGE and HPLC. SPECIFIC ACTIVITY > 17U/mg (one unit binds 1 μg Dbiotin at pH 8.9). PROTEOLYTIC ACTIVITY < 10 U/mg protein (25 °C, 24 h, pH 8.0). AMINO ACID SEQUENCE MAEAGITGTWYNQLGSTFIVTAGADGALTGTYESAVGNAESRYVLTGRYDSAPATDGSGTALGWTVAWKNNYRNAHSATTWSGQYVGGAEARINTQWLLTSGTTEANAWKSTLVGHDTFTKVKPSAAS. USAGE PuriMag’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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1. Hailiang Sun, Jiate Lin, Zhiting Liu, Yanan Yu, Meihua Wu, Shuo Li, Yang Liu, Yaling Feng, Yuqian Wu, Mingliang Li, Peirong Jiao, Kaijian Luo and Ming Liao. Genetic, Molecular, and Pathogenic Characterization of the H9N2 Avian Influenza Viruses Currently Circulating in South China, Viruses 2019, 11(11), 1040 （链霉亲和素包覆多孔板）
2. Yang Liu, Shuo Li, Huapeng Sun, Liangqi Pan, Xinxin Cui, Xuhui Zhu, Yaling Feng, Mingliang Li, Yanan Yu, Meihua Wu, Jiate Lin, Fengxiang Xu, Shaohua Yuan, Shujian Huang, Hailiang Sun and Ming Liao. Variation and Molecular Basis for Enhancement of Receptor Binding of H9N2 Avian Influenza Viruses in China Isolates, Front. Microbiol., 17 December 2020, https://doi.org/10.3389/fmicb.2020.602124 （链霉亲和素包覆多孔板）
3. Meihua Wu, et al. Molecular Characteristics, Antigenicity, Pathogenicity, and Zoonotic Potential of a H3N2 Canine Influenza Virus Currently Circulating in South China, Front. Microbiol., 09 March 2021, doi: 10.3389/fmicb.2021.628979 （链霉亲和素包覆多孔板）
4. 林嘉特. 2017～2019年华南地区H9N2 AIV对哺乳动物的致病性研究[D].华南农业大学,2020.DOI:10.27152/d.cnki.ghanu.2020.000966. 5. 于亚南. 2017～2019年广东省猪流感病毒遗传演化、受体特性及致病性分析[D].华南农业大学,2020.DOI:10.27152/d.cnki.ghanu.2020.001531.
